Hbss or other media containing 30% serum fcs method. During operation, the instruments spinning action tilts the shandon cytofunnels upright and centrifuges cells onto the deposition area of the slide, giving all. Thermo shandon cytospin 3 manual shandon centrifuges the shandon cytospin 3 centrifuge offers excellent preparations. Cytospin collection fluid is an excellent fixative for all cytologic specimens. By continuing to browse the site you are agreeing to our use of cookies. As soon as possible after the shandon cytospin has stopped spinning, remove the sealed head from the instrument and open it in a biological safety cabinet. Protocol for the preparation of urine and nonbloody specimens. Histologistics instrument operating procedure shandon cytospin 4.
Cytospin 4 cytocentrifuge, cytospin 4 thermo fisher scientific. Thermo scientific shandon cytospin 4 cytocentrifuge. The s handon cytospin 3 was the first centrifuge to use lowspeed centrifugal forces to separate and deposit monolayer of cells on slides, the cytospin 3 centrifuge helps maintain cellular integrity. Thermo scientific cytospin 4 cytocentrifuge histololgy.
Shandon cytospin 4 cytocentrifuge the ultimate thinlayer cell preparation system shandon cytospin 4 cytocentrifuge the cytospin has been synonymous with cytocentrifugation for over 35 years. The thermo shandon cytospin 3 is a low speed centrifuge used to separate and deposit a monolayer of cells on slides while maintaining cellular integrity. Detection of leukemic lymphoblasts in csf is instrumentdependent. The thermo scientific cytospin name has been synonymous with cytocentrifugation since the first launch in 1967. Service technicians pathology forum shandon cytospin 4 centrifuge service manual luz ortiz ciracet corp hello. Shandon cytospin 3 centrifuge newest vintage with balance detectionrotor assembly user manual pdf on request the shandon cytospin 3 cytocentrifuge was the first device to use lowspeed centrifugal force to separate and deposit a monolayer of cells on slides while maintaining cellular integrity. Thermo scientific cytospin 4 cytocentrifuge, cytospin 4. The sensitivity of thinprep and cytospin cytology was increased with increasing number, size, pathological stage, and grade, but there was no significant difference between the two tests. Detection of leukemic lymphoblasts in csf is instrument.
The shandon cytospin centrifuge is designed to protect the operator before, during and after sample preparation. Read free thermo shandon processor manual search and download pdf files for free. Service technicians forum shandon cytospin 3 centrifug service manual omer kurt gateelektronik i need the shandon cytospin 3 centrifug service manual can you help me. Jan 26, 2017 thermo shandon cytospin 3 centrifuge available from the lab world group. This reliable benchtop centrifuge provides economical thinlayer preparations from any liquid matrix, especially hypocellular fluids such as spinal fluid and urine. Facilities storing or utilizing this material should be equipped with an eyewash facility and a safety shower. Diesel exhaust particulate material expression of in vitro. Any cs centrifuge series components such as funnels, filter cards and slides are compatible to the thermo fisher shandon cytospin series. Resuspend cell preparations in hbss containing 30% fcs to a concentration of approximately 5x105 cellsml. Thermo scientific shandon cytospin 4 operation manual pdf. University of michigan property disposition medicallab.
Pipette the appropriate amount of cell suspension 100 500 ml into each funnel. Shandon cytospin 4 standard workflow diagram kuperwasser lab. I need ther service manual for the shandon cytospin 4. The service manual for the cytospin 3 bench top centrifuge is designed to provide the locally based engineer with all the information he needs to install and maintain the cytospin in good running order.
Shandon cytospin collection fluid related applications. The studies of the patient samples indicated that the wescor cytopro was much more efficient at centrifuging cells onto a glass slide compared with the shandon cytospin 4. Accepts all protocols fromshandon cytospin 1, 2, and 3. Of the 3,085 patients who were suspected of having bladder cancer, 379 had an established diagnosis of bladder cancer by transurethral resection of the bladder tumor. Jun 16, 2014 these 3,085 patients underwent urinary cytology using both thinprep and cytospin. For the thermo scientific shandon cytospin centrifuge. Shandon cytospin 3 clips not included, but available from block scientific. Sealed cover protects user from exposure to aerosoling pathogenic samples. Assemble cytoblock cassette into thermo scientific shandon cytoclip, with cytoclip horizontal. Cytospin preparation of the cerebrospinal fluid of case openi.
Thermo scientific shandon cytospin collection fluid. The best cell recovery occurred with our current shandon cytospin 4 setting figure 1. Cytospin 4 manual usc school of pharmacy core facilities. Use adequate ventilation to keep airborne concentrations low. Introduction this procedure describes the test which can be carried out, at any time, on the cytospin 3, to ascertain if the machine is functioning correctly.
Cytospin preparation of the cerebrospinal fluid of case. Apply 3 drops of reagent 1 into the center of the well in the board insert. May 01, 2016 shandon cytospin 3 service manual readdownload the thermo scientific cytospin 4 cytocentrifuge is an ultralight. Cytospin 3 centrifuge from shandon is useful in application areas such as hematologyoncology, cytologyhistology, rheumatology, virologyserology, microbiology, and immunochemistry. White filter card is preattached and is designed for singleuse only. Thermo shandon cytospin 3 available from the lab world group. Shandon cytospin 3 service manual readdownload the thermo scientific cytospin 4 cytocentrifuge is an ultralight. Shandon cytospin collection fluid material safety data sheet page 4 of 4 september 17, 2003 tsca usa toxic substance control act sara title iii usa superfund amendments and reauthorization act section 15 regulatory information the information contained herein is believed to be accurate but is not warranted to be so. Histologistics instrument operating procedure shandon.
Pop up metal knob on centrifuge cover and remove lid. More than 15,000 laboratories worldwide use this simple, reliable instrument. Dec 23, 2014 service technicians pathology forum shandon cytospin 4 centrifuge service manual luz ortiz ciracet corp hello. Thermo shandon limited is an iso 9001 and tickit accredited company. Pdf analysis of urine cytology tests in 120 paired cases. Instruments required one multimeter, one 1ooov megger, one stopwatch. Shandon cytospin 3, cell preparation system, life sciences international europe, 31997. There are no safety issues associated with the cytospin when used properly. Functional equivalent of the shandon single cytofunnel fully compatible with shandon cytospin centrifuge. Packaged in convenient pump bottle or prefilled specimen cups.
Instrument operating procedure shandon cytospin 4 written by hans b snyder 5. Connect power plug the power supply into a grounded outlet supplying the voltage and frequency indicated on the power supply. You must have previously filled out a project information sheet. Thermo scientific shandon cytospin 4 operation manual.
It is essential that the maintenance routines are followed so that the. Jun 24, 2016 thermo shandon cytospin 3 available from the lab world group. A comparison between thinprep monolayer and cytospin cytology. Thermo scientific shandon cytospin 3 cytospinservice. The lid remains locked at all timesduring rotation. Description pkgqty order number shandon cytospin sealed head each 59910018. A comparison between thinprep monolayer and cytospin. Specimen should be fixed prior to beginning cytoblock preparation in the cytospin.
The overall specificity of thinprep and cytospin cytology was 94. Brown filter card for slower absorption of sparse samples ideal for csf. Description the sealed head cytoclip slide clip inner bowl lid assembly locking device kit ball locking device body sealed head handle assembly seal screwm3x8 moulded lid sealed head hub sealed head seal kit each sealed head requires 3 different seals. If necessary balance by picking up front of machine and gently replacing on counter. Simultaneously spinning up to 24 samples in 12 chambers, this shandon centrifuge uses microtitre strips. The sealed cover protects the user from exposure to aerosoling pathogenic samples. You will be bidding on a thermo scientific shandon cytospin 4. This item powers on but it may be missing parts and the overall operating condition is unknown. Fisherbrand single cytology funnels print share fisherbrand single cytology funnels.
It provides economical thinlayer preparations from any liquid matrix. Thermo shandon processor manual citadel 2000 mobi thermo shandon processor manual citadel 2000 as recognized, adventure as. Shandon cytospin 3 centrifuge shandon cytospin iii cytofuge. Cytofunnels load in a titled position preventing cell loss. Thermo scientific shandon cytospin 3 cytospinservice manual. Make sure that the detail on the carton corresponds with the purchase order before the cytospin is removed from its carton. When power is connected, two beeps will sound and the cover lock will release.
Thermo shandon cytospin 3 centrifuge available from the lab world group. Thermo scientific shandon cytospin 4 cytocentrifuge thermoshandonprocessormanualcitadel2000 11 pdf drive page 311. Shandon cytospin 4 centrifuge service manual service. Instrument operating procedure shandon cytospin 4 written by hans b snyder vii. Preparation of cells for microscopy using cytospin. As an added safety measure, the shandon cytospin will only operate when the lid is locked into position. Store specimens or unstained slides for weeks without adverse effect with thermo scientific shandon cytospin collection fluid. The cytospin is under warranty with thermo fisher and has a service record containing information about all previous repairs on the equipment. You can add community subscriptions in the search bar that says subscribe to more communities. These 379 patients consisted of 314 men and 65 women, whose mean age was 65. In the following tests a sealed head is fitted without cell or cell holders. Numerous red blood cells are seen in the background wrightgiemsa stain. Shandon cytospin 3, cell preparation system, life sciences international europe, 3 1997. Sep 02, 2015 unload cytospin sample chambers page 97 the shandon cytospin 4 lid will unlock automatically as soon as the shandon cytospin has stopped spinning.
Position the cytofuge 2 away from direct sunlight and sources of heat or cold. Redesigned lidrelease mechanism allows for onehanded opening and closingof the outer lid for easy access. Cytospin preparation of the cerebrospinal fluid of case 1 showing promyelocytes with auer rods in the cytoplasm. The locating peg on the back of the cytoblock cassette fits into the hole in the cytoclip to ensure proper orientation. Readytouse alcoholcarbowax peg combination for direct specimen collection and fixation.
Shandon cytospin 3 centrifug service manual service. Gain all the advantages of the ultimate thinlayer cell preparation system with the thermo scientific cytospin 4 cytocentrifuge. This property was used and turned in by the medical university of south carolina and is located at the. It accepts all protocols from the shandon cytospin 1, 2 and 3. Each sealed head requires 3 different seals includes 1 of each type of seal required cone seal lid seal oring seal support plate thumb nut part number 59910052 59920046 74010103 74010104 p07437 59930093 59910019 this pack 59920047 59930098 quantity specification and accessories page reference 65 66 66 66 65 65 appendix c. Biomarkers such as epithelial membrane antigen ema, glucose transporter1 glut1, and insulinlike growth factorii mrnabinding protein 3 imp3 are useful in separating benign cells from malignant cells. Accepts all protocols from shandon cytospin 1, 2, and 3. Shandon cytospin 4 cytocentrifuge the ultimate thinlayer cell preparation system. The used cytofuge, shandon cytospin 3 cytofuge is a low speed centrifuge used to separate and deposit a monolayer of cells on slides while maintaining cellular integrity. Thermo scientific shandon cytoblock cell block preparation. The number one thing to remember when using the cytospin is that the rotor must be removed from the centrifuge unit before opening or closing the cover on the rotor.
Cytospin instructions these are general guidelines to help remind users who have already been trained 1. As an added safety measure, the shandon cytospin4 will only operate when the lid is locked into position. If we know the equipment is not working, we will list it on the form. For assistance in choosing cytospin accessories and selecting the program settings for the cytospin, either contact. Redesigned lidrelease mechanism allows for onehanded opening and closing of the outer lid for easy access. Shandon cytospin 3 iii cyto centrifuge cytocentrifuge. Shandon cytospin collection fluid thermo fisher scientific. Thinprep slides were compared to direct smears in 79 cases. Concentrate the fixed cells by centrifugation, pour off excess fluid and drain.
The rotor is very delicate and must be handled with care. Conventional liquidbased techniques versus cytyc thinprep. For more information, please visit us at shandon cytospin 3. However, immunocytochemistry performed on cytospin cells helps to differentiate these cells. Shandon cytospin 3 cytocentrifuge the lab world group. To provide a method for the preparation of cytospins from cell suspensions.
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